Enzymes and Inhibitors

931 – 945 of 5,374 results

931

Promega T3 RNA Polymerase

DNA-dependent RNA polymerase for in vitro transcription.

Pricing & Availability
Specifications

Components	100mM DTT 5X Reaction Buffer, transcription optimized 5X Buffer: 200mM Tris HCl (pH 7.9 at 25°C), 30mM MgCl2, 10mM Spermidine, 50mM NaCl
Content And Storage	-30°C to -10°C
Form	Liquid
Product Type	T3 RNA Polymerase
Storage Buffer	20mM Potassium Phosphate (pH 7.7 at 25°C), 1mM EDTA, 10mM DTT, 100mM NaCl, 0.1% Triton™ X-100, 50% Glycerol
Color	Colorless
pH	7.7
Purity	>90%
For Use With (Application)	Generation of templates for in-vitro translation, generation of probes for Nucleic Acid hybridization, generation of RNA processing Substrates, generation of Antisense RNA
Source	Recombinant E. coli strain

932

Promega Modifying Enzymes: Single-Stranded DNA Binding Protein

Binds with high affinity in a cooperative manner to single-stranded DNA but does not bind well to double-stranded DNA

Pricing & Availability

933

Promega T4 DNA Ligase Buffer Pack

For ligation of DNA ends.

Pricing & Availability

934

Promega T4 DNA Ligase

For ligation of DNA ends.

Pricing & Availability
Specifications

Components	10X Reaction Buffer: 300mM Tris HCl (pH 7.8 at 25°C), 100mM MgCl2, 100mM DTT and 10mM ATP
Content And Storage	-30°C to -10°C
Form	Liquid
Product Type	T4 DNA Ligase
Storage Buffer	10mM Tris HCl (pH 7.4 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 50% Glycerol
Molecular Weight (g/mol)	68
Color	Colorless
pH	7.4
Purity	>90%
For Use With (Application)	Joining double-stranded DNA molecules with cohesive or blunt ends (5', 3' or blunt-ended DNA inserts)
Source	Recombinant E. coli strain

935

Promega Western Blue™ Stabilized Substrate for Alkaline Phosphatase

Stable, ready-to-use substrate for Western blots and immunoscreening.

Pricing & Availability

936

Promega Terminal Deoxynucleotidyl Transferase

TdT catalyzes the addition of mononucleotides to the terminal 3'-OH of a DNA initiator accompanied by the release of inorganic phosphate.

Pricing & Availability
Specifications

Components	5X Reaction Buffer: 500mM Cacodylate Buffer (pH 6.8 at 25°C), 5mM CoCl2, 0.5mM DTT
Content And Storage	-30°C to -10°C
Form	Liquid
Product Type	Terminal Deoxynucleotidyl Transferase
Storage Buffer	50mM Potassium Phosphate (pH 6.4), 100mM NaCl, 1mM beta-mercaptoethanol, 0.1% Tween™ 20, 50% Glycerol
Color	Colorless
pH	6.4
Concentration	30 U/μL
For Use With (Application)	Tailing reactions to add complementary Homopolymer tails to DNA vectors and cDNA, 3' end-labeling, TUNEL Assays
Source	Recombinant E. coli strain

937

Promega Proteinase K

Serine protease that exhibits a very broad cleavage specificity. Useful in many molecular biology applications.

Pricing & Availability

938

Promega Restriction Enzymes - Hind III Blue/White Cloning Qualified (E)

Capable of digesting DNA in 15 minutes or less. Star activity may be observed in the presence of Mn²⁺. (A/AGCT T T TCGA\A)

Pricing & Availability
Specifications

Cut Site	A.AGCTT TTCGA.A
Form	Liquid
Product Type	HindIII
Storage Buffer	10mM Tris HCl (pH 7.4), 250mM NaCl, 0.1mM EDTA, 1mM DTT, 0.5mg/mL BSA, 50% Glycerol
Incubator Temperature	37°C
For Use With (Application)	Provides a higher level of quality control for Enzymes used in cloning applications

939

Promega Streptavidin Alkaline Phosphatase

Used for the detection of biotinylated molecules.

Pricing & Availability

940

Promega Rapid Digestion Kit---Trypsin, 100microg

The Rapid Trypsin and Rapid Trypsin/Lys-C Digestion Kits are designed to shorten protein digestion times to 60 minutes versus the typical 4-18 hours.

Pricing & Availability

941

Promega Restriction Enzymes - Nde I (D)

Active and capable of digesting DNA in 15 minutes or less, directly in GoTaq™ Green Master Mix. (CA/TA TG GT AT\AC)

Pricing & Availability

942

Promega Restriction Enzymes - Xho I Blue/White Cloning and Genome Qualified (D)

Capable of digesting DNA in 15 minutes or less. Genome qualified to ensure optimal performance in genomic analysis applications. (C/TCGA G G AGCT\C)

Pricing & Availability
Specifications

Cut Site	C.TCGAG GAGCT.C
Form	Liquid
Storage Buffer	10mM Tris HCl (pH 7.3), 300mM KCl, 0.1% Triton™ X-100, 0.1mM EDTA, 1mM DTT, 0.5mg/mL BSA, 50% Glycerol
pH	7.3
Incubator Temperature	37°C
For Use With (Application)	Provides a higher level of quality control for Enzymes used in cloning applications

943

Promega Restriction Enzymes - BamH I Blue/White Cloning Qualified (E)

Capable of digesting DNA in 15 minutes or less. Blue/white cloning qualified, providing a higher level of quality control for enzymes used in cloning applications. (G/GATC C C CTAG\G)

Pricing & Availability
Specifications

Cut Site	G.GATCC CCTAG.G
Form	Liquid
Storage Buffer	10mM Tris HCl (pH 7.4), 300mM KCl, 5mM MgCl2, 0.1mM EDTA, 1mM DTT, 0.5mg/mL BSA, 50% Glycerol
pH	7.4
Incubator Temperature	37°C
For Use With (Application)	Provides a higher level of quality control for Enzymes used in cloning applications

944

Promega Restriction Enzymes - Taq I (E)

Sensitive to overlapping dam methylation. (T/CG A A GC\T)

Pricing & Availability
Specifications

Cut Site	T.CGA AGC.T
Form	Liquid
Storage Buffer	10mM Tris HCl (pH 7.4), 300mM KCl, 0.1mM EDTA, 1mM DTT, 0.5mg/mL BSA, 50% Glycerol
pH	7.4
Incubator Temperature	37°C

945

Promega Restriction Enzymes - Sma I Blue/White Cloning and Genome Qualified (J)

XmaI is a neoschizomer of SmaI. Available at high concentration, containing 5,000 units of SmaI at a concentration of 40-80u/μL. (CCC/GGG GGG\CCC)

Pricing & Availability
Specifications

Cut Site	CCC.GGG GGG.CCC
Form	Liquid
Storage Buffer	10mM Tris HCl (pH 7.4), 300mM KCl, 0.1mM EDTA, 1mM DTT, 0.5mg/mL BSA, 50% Glycerol
pH	7.4
Incubator Temperature	37°C
For Use With (Application)	Provides a higher level of quality control for Enzymes used in cloning applications

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